The End

This week was a stressful but successful week. I say this because I was able to finish my project for the ASU conference which is only a week away! But I have to say it was tricky trying to get all my information neatly organized. Plugging the information was the easy part of this project, but making it all fit took so long. Playing with the colors, font, font size, and all the effects kept me entertained for a while. I was able to finish this over the weekend and on Monday when I came in Matt nicely went over it and gave me some feedback. I was able to change a few things to make it better and even though I personally like the pink color I originally chose that  looked cute, I changed it to a lighter pink because the one I had was too bright. Anyway, since my project is finished and Matt has already gone over it I want to see if all of you would be willing to give me some feedback on my project. It is in our dropbox for all of you who want to check it out.

Since my project is done with I think it is only fair to share with you what my conclusions were. Let me catch you all up with what my purpose for this project was, summarize all my results and inform you what my conclusion is.

For starters the purpose for my project was to test a variety of extraction kits and protocols that would determine the most efficient method for running PCR. 

I tested three different extraction kits;

• Epicentre: QuickExtract Bacteria DNA Extraction,
• Protocol Isolation and Purification of total genomic DNA
• Sigma: GenElute Bacteria Genomic DNA Kit

I tested different bacteria with each kit;

• AgroBacterium (gram-negative)
• Bacillus subtilis (gram-positive)
• Escherichia coli (gram-negative)    
• Salmonella (gram-negative)           
• Staphylococcus aureus (gram-positive)
• Staphylococcus epidermidis (gram-positive)

Bacteria	Epicentre	Sigma Extractions	Isolation and Purification of genomic DNA Extractions		PCR Results
AgroBacterium tumefaciens	Positive	Positive	Negative	Positive
Bacillus subtilis	Positive	Negative	Negative	Positive
Escherichi coli	Positive	Positive	Positive	Positive
Salmonella	Positive	Negative	Negative	Negative
Staphylococcus aureus	Positive	Negative	Negative	Positive
Staphylococcus epidermidis	Positive	Negative	Negative	Negative

 The table you see is the same table I included in my poster, this table shows which kits and protocols worked for each bacteria and the last column shows which bacteria had positive results after running PCR on them. If you can see the protocol that had the least amount of successful extractions was the Isolation and Purification of genomic DNA, while Epicentre had the most successful extractions and the Sigma worked for the gram-negative bacteria.