After a long break, I can honestly say I am glad to be back.
I am ready for what is my last semester at Phoenix College, and excited to
start my internship with Matt and Josh.
Today was my first day back and because I applied for the
MGE Conference for ASU my advisers and I thought it would be a good idea to
redo all of my experiments that were done last semester. For those of you who
have no idea what my project consist of it has to do with finding the most
efficient method for running a PCR. Efficiency is determined by several
variables, including cost, quantity and quality of DNA, time required for
extraction, and which method produced clear PCR product. In order to be able to
run a PCR I first have to successfully extract DNA from bacteria. With that my
job today was to make cultures for the several different bacteria that I will
be using in both the Quick Extract Bacterial DNA Extraction Kit Protocol and
the Isolation and Purification of Total Genomic DNA Protocol.
 | |
| Rack where Bacteria is stored |
 |
| Rack with TSB tubes |
Procedures
 | ||||||||||||||
My four cultures
|
Before anything was done, I made sure to label each TSB tube
to its corresponding bacteria. The 4 TSB tubes are needed because I have to add a few drops of
bacteria in each, helping the bacteria grow. However before adding these drops
into the TSB tubes each bacteria was gently pipetted allowing the bacteria to
mix, we do this to get rid of the bacteria clumps that are located at the
bottom of the tubes. After mixing it well two drops of each bacteria was added
to its corresponding TSB tube. These TSB tubes were all covered and incubated.
They have to be left in the incubator for a whole night to allow the bacteria
to grow in order to use them for the DNA extraction experiments.
Materials
E. Coli
S. Aureus
Bacillus
Agrobacterium
4 TSB Tubes
Congrats on your last semester here at PC, the brave new world awaits you!! This is a really great experiment that you are working on. The first project that I had when I started my Bioscience internship was PCR and I was thrilled to see my results...even if it didn't quite work as I had hoped. The E.Coli bacteria (stock or otherwise) was not running like it was supposed to at the last stage - electrophoresis. I was able to see my DNA (it glowed) but it didn't run along the gel as my ruler did. I hope that you have better success with your experiment and I'd love to take a look at your finished results when your project is complete. Good luck!
ReplyDeleteThis comment has been removed by the author.
ReplyDelete